Publications

2019
Wondafrash M, Slippers B, Hurley BP, Garnas JR. Local antagonism and resource partitioning among two invasive pine plantation pest. Agricultural and Forest Entomology. 2019;21 :276-285.
Fitza KNE, Garnas JR, Lombardero MJ, Ayres MP, Krivak-Tetley FE, Ahumada R, Hurley BP, Wingfield MJ, Slippers B. The global diversity of Deladenus siricidicola in native and non-native populations. Biological Control. 2019;132 :57-65.
Queffelec J, Wooding A, Greeff J, Garnas JR, Hurley BP, Wingfield MJ, Slippers B. Potential mechanisms that influence sex ratio variation in the invasive Hymenopteran Sirex noctilio in South Africa. Ecology and Evolution. 2019;14 :7966-7973.
Mutitu EK, Hoareau TB, Hurley BP, Garnas JR, Wingfield MJ, Slippers B. Reconstructing routes of invasion of the Bronze Bug Thaumastocoris peregrinus ﴾Hemiptera: Thaumastocoridae﴿. Biological Invasions. 2019;In press.
2018
Burgess T, McDougall K, Scott P, Hardy G, Garnas J. Predictors of Phytophthora diversity and community composition in natural areas across diverse Australian ecoregions. Ecography [Internet]. 2018;42 (1) :1-14. Publisher's VersionAbstract
Comprehensive understanding of the patterns and drivers of microbial diversity at a landscape scale is in its infancy, despite the recent ease by which soil communities can be characterized using massively parallel amplicon sequencing. Here we report on a comprehensive analysis of the drivers of diversity distribution and composition of the ecologically and economically important Phytophthora genus from 414 soil samples collected across Australia. We assessed 22 environmental and seven categorical variables as potential predictors of Phytophthora species richness, αandβ diversity, including both phylogenetically and non-phylogenically explicit methods. In addition, we classified each species as putatively native or introduced and examined the distribution with respect to putative origin. The two most widespread species, P. multivora and P. cinamomi, are introduced, though five of the ten most widely distributed species are putatively native. Introduced taxa comprised over 54% of Australia's Phytophthora diversity and these species are known pathogens of annual and perennial crop habitats as well as urban landscapes and forestry. Patterns of composition were most strongly predicted by bioregion (R2=0.29) and ecoregion (R2=0.26) identity; mean precipitation of warmest quarter, mean temperature of the wettest quarter and latitude were also highly significant and described approximately 21%, 14% and 13% of variation in NMDS composition, respectively. We also found statistically significant evidence for phylogenetic over-dispersion with respect to key climate variables.This study provides a strong baseline for understanding biogeographical patterns in this important genus as well the impact of key plant pathogens and invasive Phytophthora species in natural ecosystems.
Burgess TI, Tan YP, Garnas J, Edwards J, Scarlett KA, Shuttleworth LA, Daniel R, Dann EK, Parkinson LE, Dinh Q, et al. Current status of the Botryosphaeriaceae in Australia . Australasian Plant Pathology [Internet]. 2018. Publisher's VersionAbstract

The Botryosphaeriales, and in particular the Botryosphaeriaceae, are a well-studied group of fungi best known for the canker diseases they cause on woody hosts especially in stressed or damaged trees. Australian Plant Pathology herbaria contain many records for this group, but due to considerable taxonomic changes over the past decade, many of the species names have since been reclassified. In this article we used all published records with available sequence data of the Botryosphaeriaceae in Australia to examine the distribution and host range of these taxa. There are 24 genera encompassing 222 species in the Botryosphaeriaceae; 9 genera and 62 species have been recorded in Australia. Some genera such as Neoscytalidium are only found in warm, humid climates while Dothiorella species are more common in temperate climates. There were species, such as Lasiodiplodia theobromae, Neofusicoccum parvum and Botryosphaeria dothidea, which had a wide host range with many records. However, there were also several species found only in one location on a single host. While systematic data collection is still required, the information presented here provides a baseline of species present in Australia and will underpin future studies into this group of important pathogens.

Asbjornsen H, Campbell JL, D'Amato AW, Garnas J, Gunn JS, Iverson LR, Ontl TA, Peters MW, Shannon PD. Forest management options for addressing drought in the Midwest and Northeast U.S. Service USF. Forests and Drought. 2018.
Garnas J. Rapid evolution of insects to global environmental change: conceptual issues and empirical gaps. Current Opinions in Insect Science [Internet]. 2018;29 :93–101. LinkAbstract

Understanding how insects will respond both ecologically and evolutionarily to complex and interacting factors linked to global change is an important challenge that underpins our ability to produce better predictive models and to anticipate and manage ecosystem-scale disruption in the Anthropocene. Insects have the capacity to rapidly adapt to changing conditions via a variety of mechanisms which include both phenotypically plastic and evolutionary responses that interact in important ways. This short review comments on the current state of knowledge surrounding rapid evolution in insects and highlights conceptual and empirical gaps. Emphasis is placed on the need to consider direct and indirect community-level feedbacks via both ecological and evolutionary mechanisms when examining the consequences of global change, with particular focus on insects and their facultative and obligate symbionts.

2017
Wondafrash M, Slippers B, Garnas J, Hurley B. Parasitoid assemblage associated with a North American pine weevil in South Africa . Agricultural and Forest Entomology. 2017.Abstract

 1 The weevil Pissodessp. was first reported as an introduced pest on exotic Pinusspp.
inSouthAfricain1942.ItisonlyrecentlythatthenativewaspPycnetronpixPrinsloo
was described fromSouth Africaas a parasitoidof thisweevil.
2 We estimated the frequency and distribution of the association between P. pix and
Pissodessp., as well as the occurrence of possible other natural enemies. Parasitoids
were reared from Pissodes-infested Pinus radiata D. Don and Pinus patula Schiede
ex Schltdl. & Cham logs collected frommajor Pinus-growing regions.
3 The identity of parasitoids was confirmed using morphological and molecular tech-
niques. Parasitismwas confirmed by analyzing gut content sequences of parasitoids.
4 Pycnetron pix was found parasitizing Pissodes sp. throughout major Pinus-growing
provinces of the country. Another native parasitoid, Cratocnema sp., is reported for
the first time as a parasitoid of Pissodes sp. Rhopalicus tutela (Walker), a known
parasitoid of Pissodes spp. in their native range, was also detected and confirmed to
be ofEuropean origin.
5 Althoughcharacterizedbyanerraticdistributionandalowparasitismrate,anaccruing
suite of natural enemies was documented, suggesting that there is potential for
augmentative biological control of Pissodessp. inSouth Africa.

Burgess TI, White D, McDougall KM, Garnas J, Dunstan WA, Català S, Carnegie AJ, Worboys S, Cahill D, Vettraino A-M, et al. Distribution and diversity of Phytophthora across Australia . Pacific Conservation Biology [Internet]. 2017;23 :1-13. doi: 10.1071/PC16032Abstract

The introduction and subsequent impact of Phytophthora cinnamomi within native vegetation is one of the major conservation issues for biodiversity in Australia. Recently, many new Phytophthora species have been described from Australia’s native ecosystems; however, their distribution, origin, and potential impact remain unknown. Historical bias in Phytophthora detection has been towards sites showing symptoms of disease, and traditional isolation methods show variable effectiveness of detecting different Phytophthora species. However, we now have at our disposal new techniques based on the sampling of environmental DNA and metabarcoding through the use of high-throughput sequencing. Here, we report on the diversity and distribution of Phytophthora in Australia using metabarcoding of 640 soil samples and we compare the diversity detected using this technique with that available in curated databases. Phytophthora was detected in 65% of sites, and phylogenetic analysis revealed 68 distinct Phytophthora phylotypes. Of these, 21 were identified as potentially unique taxa and 25 were new detections in natural areas and/or new introductions to Australia. There are 66 Phytophthora taxa listed in Australian databases, 43 of which were also detected in this metabarcoding study. This study revealed high Phytophthora richness within native vegetation and the additional records provide a valuable baseline resource for future studies. Many of the Phytophthora species now uncovered in Australia’s native ecosystems are newly described and until more is known we need to be cautious with regard to the spread and conservation management of these new species in Australia’s unique ecosystems.

Olivier-Espejel S, Hurley BP, Garnas J. Assessment of beetle diversity, community composition and potential threats to forestry using kairomone-baited traps. Bulletin of Entomological Research [Internet]. 2017;107 :106-117. doi: 10.1017/S0007485316000699Abstract

Traps designed to capture insects during normal movement/dispersal, or via attraction to non-specific (plant) volatile lures, yield by-catch that carries valuable information about patterns of community diversity and composition. In order to identify potential native/introduced pests and detect predictors of colonization of non-native pines, we examined beetle assemblages captured in intercept panel traps baited with kairomone lures used during a national monitoring of the woodwasp, Sirex noctilio, in Southern Africa. We identified 50 families and 436 morphospecies of beetles from nine sites sampled in both 2008 and 2009 and six areas in 2007 (trap catch pooled by region) across a latitudinal and elevational gradient. The most diverse groups were mainly those strongly associated with trees, known to include damaging pests. While native species dominated the samples in terms of richness, the dominant species was the introduced bark beetle Orthotomicus erosus (Curculionidae: Scolytinae) (22 +/- 34 individuals/site). Four Scolytinae species without previous records in South Africa, namely Coccotrypes niger, Hypocryphalus robustus (formerly Hypocryphalus mangiferae), Hypothenemus birmanus and Xyleborus perforans, were captured in low abundances. Communities showed temporal stability within sites and strong biogeographic patterns across the landscape. The strongest single predictors of community composition were potential evaporation, latitude and maximum relative humidity, while the strongest multifactor model contained elevation, potential evaporation and maximum relative humidity. Temperature, land use variables and distance to natural areas did not significantly correlate with community composition. Non-phytophagous beetles were also captured and were highly diverse (32 families) perhaps representing important beneficial insects.

Mbenoun M, Garnas JR, Wingfield M, Boyogueno A, Roux J. Metacommunity analyses of Ceratocystidaceae fungi across heterogeneous African savanna landscapes . Fungal Ecology [Internet]. 2017;28 :76-85. Publisher's VersionAbstract

Metacommunity theory offers a powerful framework to investigating the structure and dynamics of ecological communities. We use Ceratocystidaceae fungi as an empirical system to explore the potential of metacommunity principles to explain the incidence of putative fungal tree pathogens in natural ecosystems. The diversity of Ceratocystidaceae fungi was evaluated on elephant-damaged trees across the Kruger National Park of South Africa. Multivariate statistics were then used to assess the influence of landscapes, tree hosts and nitidulid beetle associates as well as isolation by distance on fungal community structure. Eight fungal and six beetle species were recovered on trees representing several plant genera. The distribution of Ceratocystidaceae fungi was highly heterogeneous across landscapes. Both tree host and nitidulid vector emerged as key factors contributing to this heterogeneity, while isolation by distance showed little influence. Our results are consistent with a model of metacommunity dynamics combining species sorting and patch dynamics processes.

2016
Garnas JR, Auger-Rozenberg MA, Roques A, Bertelsmeier C, Wingfield MJ, Saccaggi DL, Roy HE, Slippers B. Complex patterns of global spread in invasive insects: eco-evolutionary and management consequences. Biological Invasions [Internet]. 2016;18 :935-952. doi: 10.1007/s10530-016-1082-9Abstract

The advent of simple and affordable tools for molecular identification of novel insect invaders and assessment of population diversity has changed the face of invasion biology in recent years. The widespread application of these tools has brought with it an emerging understanding that patterns in biogeography, introduction history and subsequent movement and spread of many invasive alien insects are far more complex than previously thought. We reviewed the literature and found that for a number of invasive insects, there is strong and growing evidence that multiple introductions, complex global movement, and population admixture in the invaded range are commonplace. Additionally, historical paradigms related to species and strain identities and origins of common invaders are in many cases being challenged. This has major consequences for our understanding of basic biology and ecology of invasive insects and impacts quarantine, management and biocontrol programs. In addition, we found that founder effects rarely limit fitness in invasive insects and may benefit populations (by purging harmful alleles or increasing additive genetic variance). Also, while phenotypic plasticity appears important post-establishment, genetic diversity in invasive insects is often higher than expected and increases over time via multiple introductions. Further, connectivity among disjunct regions of global invasive ranges is generally far higher than expected and is often asymmetric, with some populations contributing disproportionately to global spread. We argue that the role of connectivity in driving the ecology and evolution of introduced species with multiple invasive ranges has been historically underestimated and that such species are often best understood in a global context.

Hui C, Richardson DM, Landi P, Minoarivelo HO, Garnas JR, Roy HE. Defining invasiveness and invasibility in ecological networks. Biological Invasions [Internet]. 2016;18 :971-983. doi: 10.1007/s10530-016-1076-7Abstract

The success of a biological invasion is context dependent, and yet two key concepts—the invasiveness of species and the invasibility of recipient ecosystems—are often defined and considered separately. We propose a framework that can elucidate the complex relationship between invasibility and invasiveness. It is based on trait-mediated interactions between species and depicts the response of an ecological network to the intrusion of an alien species, drawing on the concept of community saturation. Here, invasiveness of an introduced species with a particular trait is measured by its per capita population growth rate when the initial propagule pressure of the introduced species is very low. The invasibility of the recipient habitat or ecosystem is dependent on the structure of the resident ecological network and is defined as the total width of an opportunity niche in the trait space susceptible to invasion. Invasibility is thus a measure of network instability. We also correlate invasibility with the asymptotic stability of resident ecological network, measured by the leading eigenvalue of the interaction matrix that depicts trait-based interaction intensity multiplied by encounter rate (a pairwise product of propagule pressure of all members in a community). We further examine the relationship between invasibility and network architecture, including network connectance, nestedness and modularity. We exemplify this framework with a trait-based assembly model under perturbations in ways to emulate fluctuating resources and random trait composition in ecological networks. The maximum invasiveness of a potential invader (greatest intrinsic population growth rate) was found to be positively correlated with invasibility of the recipient ecological network. Additionally, ecosystems with high network modularity and high ecological stability tend to exhibit high invasibility. Where quantitative data are lacking we propose using a qualitative interaction matrix of the ecological network perceived by a potential invader so that the structural network stability and invasibility can be estimated from the literature or from expert opinion. This approach links network structure, invasiveness and invasibility in the context of trait-mediated interactions, such as the invasion of insects into mutualistic and antagonistic networks.

Hajek AE, Hurley BP, Kenis M, Garnas JR, Bush SJ, Wingfield MJ, Lenteren JCV, Cock MJW. Exotic biological control agents: a solution or contribution to arthropod invasions?. Biological Invasions [Internet]. 2016;18 :953-969. doi: 10.1007/s10530-016-1075-8Abstract

Biological control is a valuable and effective strategy for controlling arthropod pests and has been used extensively against invasive arthropods. As one approach for control of invasives, exotic natural enemies  from the native range of a pest are introduced to areas where control is needed. Classical biological control began to be used in the late 1800s and its use increased until, beginning in 1983, scientists began raising significant concerns and questions about nontarget and indirect effects that can be caused by these introductions. In recent years, similar issues have been raised about augmentative use of exotic natural enemies. Subsequently, international guidelines, national regulations and scientific methods being used for exotic natural enemies in biological control have changed to require appropriate specificity testing, risk assessment and regulatory oversight before exotic natural enemies can be released. National and international standards aimed at minimizing risk have increased awareness and promoted more careful consideration of the costs and benefits associated with biological control. The barriers to the implementation of classical and augmentative biological control with exotic natural enemies now are sometimes difficult and, as a consequence, the numbers of classical biological control programs and releases have decreased significantly. Based in part on this new, more careful approach, classical biological control programs more recently undertaken are increasingly aimed at controlling especially damaging invasive arthropod pests that otherwise cannot be controlled. We examine evidence for these revised procedures and regulations aimed at increasing success and minimizing risk. We also discuss limitations linked to the apparent paucity of post-introduction monitoring and inherent unpredictability of indirect effects.

Fitza KNE, Tabata M, Kanzaki N, Kimura K, Garnas J, Slippers B. Host specificity and diversity of Amylostereum associated with Japanese siricids. Fungal Ecology [Internet]. 2016;24 :76-81. doi: 10.1016/j.funeco.2016.08.005Abstract

The mutualism between siricid woodwasps and Amylostereum fungal symbionts has long been considered to be species-specific. Recent studies from North America have challenged this assumption, where native siricids and the introduced Sirex noctilio are clearly swapping symbionts. Whether this pattern is a consequence of invasion or an underappreciated property of siricid biology is unknown. Here we show that the native Japanese siricid, Sirex nitobei, carries both Amylostereum areolatum and Amylostereum chailletii, rather than only A. areolatum as long assumed. Furthermore, all samples from a Urocerus sp. unexpectedly carried, A. chailletii and not Amylostereum laevigatum. Vegetative compatibility group tests revealed extensive clonality, with one VCG present amongst three A. areolatum isolates and six VCGs present amongst 61 A. chailletii isolates. These results contribute to the understanding of insect-fungal fidelity in the siricid-Amylostereum association and, together with other studies, suggest that host tree influences Amylostereum species occurrence, perhaps more strongly than wasps.

Wondafrash M, Slippers B, Garnas J, Roux G, Foit J, Langor DW, Hurley BP. Identification and genetic diversity of two invasive Pissodes spp. Germar (Coleoptera: Curculionidae) in their introduced range in the southern hemisphere. Biological Invasions [Internet]. 2016;18 :2283-2297. doi: 10.1007/s10530-016-1159-5Abstract

During the first half of the twentieth century, two accidental cases of introduction of Pissodes weevils were recorded from the southern hemisphere. The weevils in South Africa were identified as the deodar weevil (Pissodes nemorensis) and those in South America as the small banded pine weevil (Pissodes castaneus). Wide distribution of the two species in their invasive range, general difficulty in identifying some Pissodes spp., and the varying feeding and breeding behaviours of the species in South Africa has necessitated better evidence of species identity and genetic diversity of both species and population structure of the species in South Africa. Barcoding and the Jerry-to-Pat region of the COI gene were investigated. Morphometric data of the South African species was analysed. Our results confirmed the introduction of only one Pissodes species of North American origin to South Africa. However, this species is not P. nemorensis, but an unrecognized species of the P. strobi complex or a hybrid between P. strobi and P. nemorensis. Only P. castaneus, of European origin, was identified from South America. We identified ten mitochondrial DNA haplotypes from South Africa with evidence of moderate genetic structure among geographic populations. Terminal leader and bole-feeding weevils did not differ at the COI locus. A single haplotype was identified from populations of P. castaneus in South America. Results of the present study will have implications on quarantine, research and management of these insect species.

Hurley BP, Garnas J, Wingfield MJ, Branco M, Richardson DM, Slippers B. Increasing numbers and intercontinental spread of invasive insects on eucalypts. Biological Invasions [Internet]. 2016;18 :921-933. doi: 10.1007/s10530-016-1081-x
Wingfield MJ, Garnas JR, Hajek A, Hurley BP, De Beer ZW, Taeru. Novel and co-evolved associations between insects and microorganisms as drivers of forest pestilence. Biological Invasions [Internet]. 2016;18 :1045-1056. doi: 10.1007/s10530-016-1084-7Abstract

Some of the most devastating diseases of trees involve associations between forest insects and microorganisms. Although a small number of native insect-microorganism symbioses can cause tree mortality, the majority of associations with tree health implications involve one or more exotic organisms. Here, we divide damaging symbioses between forest insects and microorganisms into four categories based on the native/exotic status of the species involved: (1) insect and microorganism are native; (2) insect is native, microorganism is exotic; (3) insect is exotic, microorganism is native; and (4) insect and microorganism are both exotic. For each category, we describe several well-researched examples of forest insect symbioses and discuss some of the consequences of the types of interactions within each category. We then discuss priorities for research on forest insect symbioses that could help to further elucidate patterns in the complexity of such interactions in the context of invasion biology. We argue that a nuanced understanding of insect-pathogen relationships is lacking, even for the few well-studied examples. Because novel associations between insects, microorganisms, and trees are increasing with globalization, such symbioses and their potential to negatively impact forest ecosystems demand focused research in the future.

Hill MP, Bertelsmeier C, Clusella-Trullas S, Garnas JR, Robertson MP, Terblanche JS. Predicted decrease in global climate suitability masks regional complexity of invasive fruit fly species response to climate change. Biological Invasions [Internet]. 2016;18 :883-891. doi: 10.1007/s10530-016-1078-5Abstract

Climate change affects the rate of insect invasions as well as the abundance, distribution and impacts of such invasions on a global scale. Among the principal analytical approaches to predicting and understanding future impacts of biological invasions are Species Distribution Models (SDMs), typically in the form of correlative Ecological Niche Models (ENMs). An underlying assumption of ENMs is that species–environment relationships remain preserved during extrapolations in space and time, although this is widely criticised. The semi-mechanistic modelling platform, CLIMEX, employs a top-down approach using species ecophysiological traits and is able to avoid some of the issues of extrapolation, making it highly applicable to investigating biological invasions in the context of climate change. The tephritid fruit flies (Diptera: Tephritidae) comprise some of the most successful invasive species and serious economic pests around the world. Here we project 12 tephritid species CLIMEX models into future climate scenarios to examine overall patterns of climate suitability and forecast potential distributional changes for this group. We further compare the aggregate response of the group against species-specific responses. We then consider additional drivers of biological invasions to examine how invasion potential is influenced by climate, fruit production and trade indices. Considering the group of tephritid species examined here, climate change is predicted to decrease global climate suitability and to shift the cumulative distribution poleward. However, when examining species-level patterns, the predominant directionality of range shifts for 11 of the 12 species is eastward. Most notably, management will need to consider regional changes in fruit fly species invasion potential where high fruit production, trade indices and predicted distributions of these flies overlap.

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