Abstract:

In the emerging era of Next Generation (NextGen) technologies, molecular markers will continue to play important roles in research requiring targeted genotyping rather than global sequencing. Using chloroplast DNA (cpDNA) markers, we generated the first evidence confirming that cpDNA is maternally inherited in Fragaria, and defined likely diploid sources of the chloroplast genomes harbored by the Fragaria hexaploid, octoploid, and decaploid allopolyploid species. As a resource for future research on Fragaria cpDNA, we used a NextGen sequencing approach to assemble a draft sequence of the Fragaria vesca subsp. americana ‘Pawtuckaway’ chloroplast genome.

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