A 445 cM long genetic linkage map consisting of seven linkage groups was constructed for the diploid (2n = 2x = 14) strawberry, Fragaria vesca. Segregation data used for linkage analysis were obtained from the F2 generation of a cross between Baron Solemacher (BS), an Alpine F. vesca variety, and WC6, an F. vesca clone collected from the wild in New Hampshire. Segregation ratios were systematically skewed in five linkage groups, in all cases favoring the BS alleies over the WC6 alleles. The 80-marker map includes 64 dominant and 11 codominant randomly amplified polymorphic DNA (RAPD) markers, an alcohol dehydrogenase locus detected as a PCR-based sequence tagged site, the phosphoglucose isomerase and shikimate dehydrogenase Isozyme loci, and the runnering and fruit color loci. A notable feature of the map is the unusually large number of codominant RAPD markers, the detection of which was due In part to the use of template mixing methods for primer testing and marker analysis. Alternate alleies of a maternally inherited RAPD marker were also detected using these methods.